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E coli Gram stain

E. coli Gram stain: Introduction, Principle, Procedure and ..

E. coli in Gram stain as shown above picture. They are bacteria which come under family Enterobacteriaceae. They are normally found in in the environment, foods, and intestines of people and animals. They are a large and diverse group of bacteria. They are normal flora when they are in our intestines E. coli are Gram-negative bacteria, meaning that they do not retain the crystal violet stain commonly used to differentiate bacteria. Their status as Gram-negative bacteria is due to their thin cell walls. E. coli has cell walls made out of two thing peptidoglycan layers, an inner and outer membrane E. coli in Gram stain showing gram negative rods having size of about 2.0 µm long and 0.25-1.0 diameter as shown above picture. Introduction of Escherichia coli E. coli is a Gram negative, aerobe and facultative anaerobic, rod-shaped bacteria. Optimal temperature for growth is 36-17°C with most strains growing over the range 18-44 °C

E coli is a gram-negative bacillus that grows well on commonly used media. It is lactose-fermenting and beta-hemolytic on blood agar. Most E coli strains are nonpigmented. The image below shows Escherichia coli on Gram staining E. coli is described as a Gram-negative bacterium. This is because they stain negative using the Gram stain. The Gram stain is a differential technique that is commonly used for the purposes of classifying bacteria The E. coli had a Gram Stain reaction color of pink and classified as Gram-negative. Both the S. epidermidis and B. subtilis had a Gram Stain reaction color of purple and then classified as Gram-positive (Table 1). Table 1: The Outcome of Gram Stain on Three Species of Bacteria Table E coli gram stain color Gram stain is the most widely used staining procedure in bacteriology. It is called a differential spot because it distinguishes between Gram-positive and Gram-negative bacteria. Bacteria that stain purple with the Gram staining procedure are ad in gram-positive; those that stain pink are said to be gram-negative E. coli is Gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane. During the staining process, E. coli picks up the color of the counterstain safranin and stains pink

Capsule - Capsules are present in some strains of the E. coli which can easily be demonstrated using India ink preparation, appear as a clear halo in a dark background. Gram Staining Reaction - Escherichia coli is a Gram -ve (Negative) bacterium. MORPHOLOGY OF ESCHERICHIA COLI (E.COLI) CULTURE REQUIREMENTS OF ESCHERICHIA COLI (E. COLI arrow_back_iosarrow_forward_ios. Q. For E-coli -Describe the Gram staining procedure -Describe how catalase test would have been done (if this test was used)- -Name all media that should have been inoculated to ID your unknown and why. arrow_forward

Morphology and Staining of Escherichia Coli: E. coli is Gram-negative straight rod, 1-3 µ x 0.4-0.7 µ, arranged singly or in pairs (Fig. 28.1). It is motile by peritrichous flagellae, though some strains are non-motile. Spores are not formed Gram Staining Escherichia Coliobserved Under Optical Stock Photo. Eisco Prepared Microscope Slide Escherichia Coli Smear Gram. Asmscience Examination Of Gram Stains Of Urine. Gram Stain Tissue Trol Control Slides From Mouse Lung Tissue. Gram Stain Of E Coli Bacterium A Gram Stain Of Shows Transcribed image text: Experiment 7 - Gram Stain Observation E. coll S. aureus C. pseudodiphtheriticum Microorganism B. cereus Shape Arrangement Cell Color Gram reaction Size um w x Diameter Drawings of a representative field on each slide: Use images from lab 7. ∞ O B. cereus S. aureus Mixture of E. coli and S. aureus E. coli C. pseudodiphtheriticum Escherichia coli* (esh-er-ee-key-ah koh. We have cultures of E. coli and Bacillus for you to gram stain. This will give you gram + and gram - controls to check your procedure against. You can use 2 slides, 1 for each bacterium, or you can divide one slide in half and smear each bacterium on the divided slide. There are also prepared, gram stained slides of bacteria of different shapes and sizes of bacteria to look at

Gram Staining of Micrococcus luteus,Escherichia coli, and Serratia marcescens INTRODUCTION: In 1884, a Danish botanist named Hans Christian Gram developed the popular staining technique known as the gram stain Escherichia coli Gram stained smear under microscopeRod shapedpink in colorthat's why Gram negative Bacilli#GramStain#GNB#GN e. coli stained gram negative under 1000x oil immersion. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features © 2021. E Coli Bacteria Coloured Scanning Electron Micrograph Sem Of. E Coli Vs S Aureus Gram Negative Vs Gram Positive Bacteria. File Bacteria Gram Stained Vaginal Smear 11 Jpg Embryology. Morphological View Of Lactococcus Culture Under Microscope 100x. Http Coltonanderson1 Weebly Com Uploads 2 4 3 0 24303411 Manual Pdf

Gram stains steps and laboratory class work procedure of E.coli and B.subtilis bacterias . Good knowledge for beginners to follow the procedure and get idea. bacilli Escherichia coli (E. coli) primary stain Bacillus subtilis (B. sub Gram negative spirillia cocci Gram positive sporulation counterstain Gram stain Staphylococcus epidermidis (S. epi) Dichotomous key mordant vegetative cell differential stain Mycobacterium smegmatis (M. smeg) endospore negative control III. INTRODUCTION

E. Coli: Gram Stain, Shape And Size Science Trend

  1. e if the organism is gram-negative, but findings do not distinguish among the other aerobic gram-negative bacilli that cause similar infectious diseases. E coli is a gram-negative bacillus that grows well on commonly used media. It is lactose-fermenting and beta-hemolytic on blood agar
  2. e their Gram Stain. After the multi-layered Gram Stain procedure each bacteria were classified as Gram-positive or Gram-negative depending on their cell walls staining color. The results showed that E. coli stained pink and classified as Gram-negative
  3. E. coli stains Gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane. During the staining process, E. coli picks up the color of the counterstain safranin and stains pink
  4. Gram Staining of Micrococcus luteus,Escherichia coli, and Serratia marcescens. INTRODUCTION: In 1884, a Danish botanist named Hans Christian Gram developed the popular staining technique known as the gram stain.His background in studying plants at the University of Copenhagen introduced him to observing different tissues under a microscope, and thus propelled his interest in pharmacology
  5. E coli gram stain results. A 38-year-old member asked: Is it possible to know the gram stain reaction without doing a gram stain in escherichia coli? Dr. Michael Ein answered. Infectious Disease 48 years experience. Yes: E. Coli is always gram negative. 5.2k views Answered >2 years ago

E. coli in gram stain: Introduction, Pathogenic strains ..

  1. THE PROCEDURE: done individually. We have cultures of E. coli and Bacillus for you to gram stain.This will give you gram + and gram - controls to check your procedure against. You can use 2 slides, 1 for each bacterium, or you can divide one slide in half and smear each bacterium on the divided slide
  2. Escherichia coli or just E. Coli, is a gram-negative rod-shaped bacteria named after Dr. Escherich Theodor, who discovered it in feces, thus concluding that it colonizes the colon.. Alright, now E. Coli is gram-negative because its cell wall has a thin peptidoglycan layer so it cannot retain the crystal violet stain, but instead, it stains pink with Safranin dye used during Gram staining
  3. Gram Staining Reaction - Escherichia coli is a Gram -ve (Negative) bacterium. MORPHOLOGY OF ESCHERICHIA COLI (E.COLI) CULTURE REQUIREMENTS OF ESCHERICHIA COLI (E. COLI) ⇒ Special requirements - Escherichia coli or E. coli have no complex nutritional requirements and readily grow in an ordinary media like Nutrient Agar medium (NAM)
  4. after gram staining, E. coli will be pink rods what color is gram positive bacteria purple which component of the gram stain procedure colors the gram-negative cells? safranin what is the purpose of using acetone-alcohol during the gram stain procedure a decolorizing agent +73 more terms. laurenbissanti. Escherichia coli

Escherichia coli Gram Stain. Gram negative bacilli - fermenter; Clinical Significance. E. coli colonizes the large intestine of humans. It is the most common pathogen of urinary tract infections and is associated with pneumonia, bacteremia (typically secondary to urinary tract infections), endocarditis, meningitis (typically in neonates), hepatic abscesses, peritonitis, endophthalmitis. E. coli serotype O157:H7 is a mesophilic, Gram-negative rod-shaped (Bacilli) bacterium, which possesses adhesive fimbriae and a cell wall that consists of an outer membrane containing lipopolysaccharides, a periplasmic space with a peptidoglycan layer, and an inner, cytoplasmic membrane

Does E coli stain Gram positive or negative

  1. Staphylococcus aureus and Escherichia coli are among the most prevalent species of gram-positive and gram-negative bacteria, respectively, that induce clinical mastitis. The innate immune system comprises the immediate host defense mechanisms to protect against infection and contributes to the initi
  2. Dr. Michael Ein answered. 48 years experience Infectious Disease. No: E. Coli is always gram negative. Send thanks to the doctor. 90,000 U.S. doctors in 147 specialties are here to answer your questions or offer you advice, prescriptions, and more. Get help now: Ask doctors free. Personalized answers. Free
  3. g, Gram-negative bacterium, usually motile by peritrichous flagella.Escherichia coli is the most common cause of acute urinary tract infections as well as urinary tract sepsis. It has also been known to cause neonatal meningitis and sepsis and also abscesses in a number of organ systems
  4. Escherichia coil is a gram negative bacterium .this is a stain negative because using the gram stain the presence of lipopolysaccharides on the outer membrane of bacteria gives it an overall negative charge to the cell wall
  5. g a gram stain on gram negative and gram positive showed that gram negative bacteria was a negative rod and that the gram positive bacteria was a positive cocci
  6. Microscopic image of E. coli after Gram staining. Based on traditional. microbiological methods, E. coli is a gram-negative rod that typically. occurs as single cells or doublets. Phylogenetically, it belongs to the. gammaproteobacterial group. Source: G.Kaiser
  7. 15.1.3.1: Escherichia coli Last updated; Save as PDF Page ID 42663; No headers. Gram Stain of Escherichia coli.Note Gram-negative (pink) bacilli. Organism. E. coli is a member of the Enterobacteriaceae family which are all Gram-negative rods; Catalase positiv

E. Coli under the Microscope Types, Techniques, Gram Stain ..

  1. Bacillus subtilis, E. coli K-12, Adeno Associated Virus-types 1-4: Risk Group 2 (RG2) Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available: Forty bacterial genera, including E.coli O157:H7; 12 fungal genera; 31 parasites; common viruses in 18 familie
  2. Escherichia coli (commonly abbreviated E.coli) is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms).Most E.coli strains are harmless, but some serotypes can cause serious food poisoning in humans
  3. e to indophenol blue (clear to blue) Oxidase +ve bacteria: Pseudomonas Vibrio. Gram stain Culture morphology Basic biochemical tests Eg Oxidase, indole, urease tests, et

The Results of Gram Stain Reactions for E

Gram Staining Of E Coli B Cereus And S Aureus. Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria, those that are gram positive, and those that are gram negative. Using a specific staining procedure, it is possible to differentiate the two types under a microscope The gram stain method of. Gram stain results determine if the organism is gram-negative, but findings do not distinguish among the other aerobic gram-negative bacilli that cause similar infectious diseases. E coli is a.

Video: Escherichia coli - Wikipedi

Morphology & Culture Characteristics of Escherichia coli

  1. Gram stains: Crystal violet, Acetone alcohol, Gram's Iodine, Safranin Acid Fast stain slides for your unknown circle on the left, and an endospore negative control (E.coli) in the circle on the right, and your unknown in the middle. The slide was heat fixed and then the endospore staining procedure was performed. 1. Take the heat fixed.
  2. ute with crystal violet staining reagent. Please note that the quality of the smear (too heavy or too light cell concentration) will affect the Gram Stain results. Wash slide in a gentle and indirect stream of tap water for 2 seconds
  3. The most widely used staining procedure in microbiology is the Gram stain, discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives
  4. . Many gene manipulation systems have been developed using E. coli as the host.
  5. In Gram-negative bacteria, the cell wall is only 1-3 layers thick , and in E. coli 80% or more of the peptidoglycan exists as a monolayer . Consistent with these earlier results, recent electron cryotomography density profile measurements have revealed that the thickness of the cell wall of both E. coli and another Gram-negative bacteria.
  6. Staphylococcus aureus and Escherichia coli are among the most prevalent species of gram-positive and gram-negative bacteria, respectively, that induce clinical mastitis. The innate immune system comprises the immediate host defense mechanisms to protect against infection and contributes to the initial detection of and proinflammatory response to infectious pathogens. The objective of the.
  7. Gram Stain (all 100X - oil immersion objective plus zoom on camera) (eyepiece = 10X; total mag. = 1000X) mixture = Gram positive and Gram negative organsims (Staphylococcus aureus and E. coli) Gram negative organisms. E.coli. Gram positive organisms. Gram + coccus . Gram + Bacillus . Gram positive rod. for comparison = yeast (unstained) at 400X.

Answered: For e-coli- Describe the Gram staining bartleb

From the observation, Escherichia coli stained red and give accurate result of Gram negative. The shape of E. coli can be observed as rod shape. Bacillus sp., Staphylococcus aureus and Actinomycetes sp. while shows results of Gram positive as all are stained with purple colour Along with gram-negative bacteria, Escherichia coli (E. coli) causes nearly 40% of nosocomial UTIs, 25% of recurrent infections, and between 80 to 90% of community-acquired infections. Proteomics, commonly used to study changes in protein expression of organisms, can be used to explore candidate biomarkers useful for the diagnosis of. Escherichia coli (E. coli)- An Overview; Enteropathogenic Escherichia coli (EPEC) Shiga toxin-producing Escherichia coli (STEC) Z-test- definition, formula, examples, uses, z-test vs t-tes Mixed E.coli & S.aureus Gram stain, 1000x Mixed growth (cocci and bacilli) on BA from unwashed hand, Gram stain, 1000x Mixed growth (Gram pos & Gram neg) on BA from mouth swab, Gram stain, 1000

그람 양성균 (gram-positive) Bacillus sp. 그람 음성균 (gram-negative) E. coli 보통 대조군으로 Bacillus 속 미생물과 E. coli 를 사용하며, 그람 양성균인지 그람 음성균인지 모르는 미지의 세균을 그람 염색할 때는 대조군들을 함께 실험에 사용하도록 합니다 E. coli stains Gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane. During the staining process, E. coli picks up the color of the counterstain safranin and stains pink ; E. coli is streptobacillus. Staphylobacillus does not exist because bacillus cells only divide across the short axis, so the.

Gram Stain

Escherichia Coli (E

The Gram staining is one of the most crucial staining techniques in microbiology. It gets its name from the Danish bacteriologist Hans Christian Gram who first introduced it in 1882, mainly to identify organisms causing pneumonia.[1] Often the first test performed, gram staining involves the use of crystal violet or methylene blue as the primary color.[2] The term for organisms that retain the. The procedure/steps of Gram Stain. Prepare and fix the specimen to the microscope slide before staining. Cover the smear with crystal violet, the primary stain, for 20 seconds. Gently rinse off the stain with water. Cover the smear with Gram's iodine, the mordant, for 1 minute. Pour off the excess Gram's iodine TEM-1 is the most commonly encountered beta-lactamase in Gram-negative bacteria. Up to 90% of ampicillin resistance in E. coli is due to the production of TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H. influenzae and N. gonorrhoeae in increasing numbers positive cells stain purple (the color of the primary, first dye used in the staining procedure), and the Gram-negative cells stain pink (the color of the counterstain, second dye used in the staining procedure). Since two different color dyes are used, the Gram stain is considered a differential stain, (i.e. it illustrates differences between. A particular strain of E. coli known as E. coli O157:H7 causes a severe intestinal infection in humans. It is the most common strain to cause illness in people. It can be differentiated from other E. coli by the production of a potent toxin that damages the lining of the intestinal wall causing bloody diarrhea

E coli 1. PRESENTED BY: NOMAN HAFEEZ KHOSA ABDUL SAMI FARHAN MISBAH SADIQ ANUM AJMAL Escherichia coli 2. 2 Esherichia coli Gram-negative rod Facultative anaerobe Named for Theodor Escherich German physician (ca. 1885) Demonstrated that particular strains were responsible for infant diarrhea and gastroenteritis Normal flora of the mouth and intestine Protects the intestinal tract from bacterial. M. tuberculosis is a bacillus and may be considered Gram-positive, but this is actually a huge misnomer. Due to the high lipid content of their unique cell wall and general stubbornness, they do not retain any portion of the Gram stain, and thus are neither truly Gram positive or Gram negative Effective utilization and understanding of the clinical bacteriology laboratory can greatly aid in the diagnosis of infectious diseases. , Although described more than a century ago, the Gram stain remains the most frequentl, The Gram stain easily divides bacteria into two groups, Gram-positive and Gram-negative, on the basis of their cell wall and cell membrane permeability Escherichia coli (commonly abbreviated E. coli) is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans Gram staining From Wikipedia, the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923, Gram-positive cocci, in purple) andEscherichia coli (Escherichia coli ATCC 11775, Gram-negative bacilli, in red), the most common Gram stain reference bacteria Gram staining (or Gram's method) is a method of.

E Coli Under Microscope Gram Stain - Micropedi

Experiment 7 - Gram Stain Observation E

E coli gram stained by my students [OC] [869x651] Close. 358. Posted by 2 years ago. Archived. E coli gram stained by my students [OC] [869x651] 21 comments. share. save. hide. report. 98% Upvoted. This thread is archived. New comments cannot be posted and votes cannot be cast. Sort by. best Figure 3.14. Gram stains. Gram stains of demonstration species. Below are shown typical Gram stain reactions of two species. E. coli (A), B. cereus old (B), B. cereus young (C). The images are slightly larger than what would be visible in a light microscope to improve clarity The Gram-negative bacilli of the genera Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter,and Proteus(Table 26- 1) are members of the normal intestinal flora of humans and animals and may be isolated from a variety of environmental sources. With the exception of Proteus, they are sometimes collectively referred to as the coliform bacilli because of shared properties, particularly. Figure 2. Live and heat-killed E. coli stained with DMAO, marking live cells (green) and EthD-III, marking dead cells (red). Figure 3. E. coli and Staphylococcus stained with CF®633 WGA (magenta) and DAPI (blue). Figure 4. Bacterial Viability and Gram Stain Kit. CF®488A WGA (green), EthD-III (red) and DAPI (blue). Live Cell Stains

Escherichia coli and Staph

E. coli are mostly harmless bacteria that live in the intestines of people and animals and contribute to intestinal health. However, eating or drinking food or water contaminated with certain. In this paper, patches of an E. coli cell wall were made using a circumferential layered model, supported by ECT imaging of both Gram-negative and Gram-positive sacculi , , plausibility arguments based on the thickness and glycan strand length , and the average peptide-peptide angle measured above (see Fig. 2B). The patches were constructed.

A Gram stain is a lab test used to detect bacteria or fungi in a sample taken from the site of a suspected infection. It gives relatively quick, preliminary results as to whether microbes are present and, if so, the general type(s) causing an infection E Coli Gram Ve Stain Https Www Facebook Com Officialmicrobiology Fref Photo Microbiology Microscopic Cells Generative Design . Gram Positive Bacteria Microbiology Microbiology Study Medical Specialties . Staphylococcus Epidermidis 1 000x 1 Microbiology Microbiology Lab Medical Laboratory Scientist Q: For e-coli- Describe the Gram staining procedure A: Ordinarily referred to as E. coli, Escherichia coli is a bacterium that is normally found in various... question_answe the Gram stain, though the bacteria are considered to be gram positive (Saviola and Bishai, 2000). Gardnella has an unusual gram-positive cell wall structure that causes bacteria of this genus to stain gram -negative or gram-variable (Sadhu et al 1989). Misinterpretation of the Gram stain has led to misdiagnosis or delaye The key difference between E. coli and Pseudomonas aeruginosa is that E. coli is a facultative anaerobic bacterial species that belongs to family Enterobacteriaceae and genus Escherichia, while P. aeruginosa is an aerobic bacterial species that belongs to family Pseudomonadadaceae and genus Pseudomonas.. Both E. coli and Pseudomonas aeruginosa are gram-negative, rod-shaped and motile bacteria

In 1922, Dorner published a method for staining endospores.Shaeffer and Fulton modified Dorner's method in 1933 to make the process faster The endospore stain is a differential stain which selectively stains bacterial endospores. The main purpose of endospore staining is to differentiate bacterial spores from other vegetative cells and to differentiate spore formers from non-spore formers Escherichia coli, également appelée colibacille et abrégée en E. coli, est une bactérie intestinale (Gram négatif) des mammifères, en forme de bâtonnet [1], très commune chez l'être humain.E. coli est une bactérie anaérobie facultative que l'on trouve dans l'intestin des vertébrés [2].En effet, elle compose environ 80 % de notre flore intestinale aérobie Differential stains- Gram stain. In contrast to simple stains, differential stains are used to distinguish the difference between bacteria. One of the most well-known differential stains is Gram stain, which differentiates gram-positive and gram-negative bacteria based on the difference in their cell wall structure.. The Gram stain was developed by the Danish bacteriologist Hans Christian Gram. Gram staining is a quick procedure used to look for the presence of bacteria in tissue samples and to characterise bacteria as Gram-positive or Gram-negative, based on the chemical and physical properties of their cell walls. The Gram stain should almost always be done as the first step in diagnosis of a bacteria infection.. The Gram stain is named after the Danish scientist Hans Christian.

The gram-negative bacterium Escherichia coli is the most numerous aerobic commensal inhabitant of the large intestine. Certain strains cause diarrhea, and all can cause infection when they invade sterile sites (eg, the urinary tract). Diagnosis is by standard culture techniques. Toxin assays may help identify the cause of diarrhea All of the previous results were than compared to gram negative unknown chart and the only bacteria that had the same results was Escherichia coli. The unknown #3 was thus identified as E. Coli. Conclusion The identity of the unknown bacterium #3, E. Coli, was supported by all of the tests that were performed for this experiment

Experiments 2 & 3 - Media & Staining - Microbiology

results of the stain should show red (Gram-negative), rod-shaped E. coli bacteria and purple (Gram-positive), coccus S. epidermidis bacteria. The Gram stain method consists of many steps; however, before beginning the staining process, a slide must be properly prepared. A sterile inoculating loop was used to transfer a single loop of sterile water onto a slide, along with a loop of each pure. E. coli are classified as facultative anaerobes, which means that they grow best when oxygen is present but are able to switch to non-oxygen-dependent chemical processes in the absence of oxygen. E. coli bacteria are gram-negative, so they stain pink in a gram test Figure 1: Gram Staining of Gram-Negative E. coli and Gram-Positive Staphylococcus Furthermore, E. coli is a commensal that produces beneficial effects such as the production of vitamin K 2 . It also prevents the colonization of the pathogenic bacteria in the intestine E. coli appeared reddish-pink which indicates that these bacteria do not bind the crystal violet stain. Therefore, it is determined to be a Gram-negative bacteria. The decolorizer extracted the lipid from outer membrane of Gram-negative bacteria washed off the crystal violet- iodine complex Gram stain: Gram-positive: Microscopic appearance: cocci in chains (liquid media) or pairs: Oxygen relationship: facultatively anaerobic bacteria: Motility: nonmotile: Catalase test: catalase-negative: Oxidase test: negative: Spores: non-spore forming: Enterococcus: Gram stain: Gram-positive: Microscopic appearance: cocci or ovoid cocci in.

Gram Stain Microbiology Lab - Lumen Learnin

Gram Staining of Micrococcus luteus, Escherichia coli, and

Gram Positive vsMicroscopy and Gram staining - YouTube

Gram stains (Värjäys) of E

Details - Public Health Image Library(PHIL)Lab Report: Isolation of Pure Culture, Gram-staining, and